General Specifications

Prometheus Panta for multi-parameter stability characterization projects

Prometheus NT.48 for protein stability characterization projects using nanoDSF technology
Prometheus NT.Plex for high throughput projects and the ability to automate with NT.Robotic Autosampler

Prometheus NT.Plex plus NT.Robotic Autosampler for unattended operation of high throughput stability characterization projects

Prometheus Panta
Prometheus NT.48
Prometheus NT.Plex
Prometheus NT.Plex + NT.Robotic Autosampler

Sample volume

10 µL

Sample handling format

Capillaries or capillary chip

Capillaries

Capillary chip

Capillary chip

Throughput in one run

Up to 48 capillaries or 24 in capillary chip

Up to 48 capillaries

24 in capillary chip

Up to 1536 in 64 capillary chips (before need to reload)

Temperature range

15 – 95 °C (up to 110 °C with High Temperature Upgrade)

15 – 95 °C

Heating rate range

Up to 95 °C: 0.1 – 7 °C/min
Above 95 °C: 0.1 – 7 °C/min

Up to 95 °C: 0.1 – 7 °C/min
Above 95 °C: 0.1 – 1 °C/min

Precision of 1 °C/min thermal ramp

± 0.2 °C

Optional upgrades

High temperature

High temperature, aggregation detection optics

High temperature, aggregation detection optics

Plate temperature control, aggregation detection optics

Optional automation

No

NT.Robotic Autosampler

N/A

21 CFR part 11 ready software

No

Yes

Dimensions

35 cm W x 51 cm H x 52 cm D

110 cm W x 188 cm H x 90 cm D
(stand-alone enclosure)

Weight

35 kg

30 kg

200 kg

Technical Specifications

Prometheus Panta
Prometheus NT.48
Prometheus NT.Plex
nanoDSF

Included

Measurement parameters

ratio: Tonset, Tm, Ea, reversibility of unfolding

330 nm: Tm

350 nm: Tm

ratio: Tonset, Tm, ΔG, ΔΔG, C50, Ea, isothermal stability, reversibility of unfolding

330 nm: Tm

350 nm: Tm

Concentration range

5 μg/mL – 250 mg/mL

Inflection point precision @ 75 °C

± 0.1 °C

Ratio precision/reproducibility

0.008

DLS

Included

N/A

Measurement parameters

Tscattering, Tsize, rH, PDI, kD, D0, average scattering intensity, reversibility of unfolding

N/A

Concentration range

0.5 mg/mL for a 15 kDa protein, up to 40% w/v

N/A

Size resolution

Down to 0.5 nm

N/A

Backreflection

Included

Optional upgrade

Measurement parameters

Tturbidity, reversibility of unfolding

Size resolution

Larger than 12.5 nm radius

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